This revised and updated second edition of Biopolymers at Interfaces builds on the success of its predecessor, featuring research contributions from nearly 60 of the profession's most distinguished international authorities. It discusses biopolymer-based multilayer structures and their application in biosensors; the progress being made in the understanding of protein behavior at the air—water interface; experimental findings in ellipsometry and reflectometry; recent developments concerning protein interfacial behavior in microfabricated total analysis systems and microarrays; surface plasmon resonance spectroscopies for protein binding studies at functionalized surfaces; and more.

From the Preface: “...In recent years, there has been continued progress in our understanding of the basics of biopolymer interfacial behavior, and new experimental methods are being developed and applied for further investigation. Furthermore, the role of biopolymer interfacial behavior in numerous applications is progressively becoming better understood, and at the same time new and exciting application areas are emerging...”

Target Audience: Surface, colloid, and interfacial scientists; biologists; biochemists; and other researchers concerned with biopolymer interfacial behavior.

Table of Contents:

Concepts:

Macromolecular Adsorption: A Brief Introduction

Driving Forces for Protein Adsorption at Solid Surfaces

Thermodynamics of Adsorption of Amino Acids, Small Peptides, and Nucleic Acid Components of Silica Adsorbents

Quantitative Modeling of Protein Adsorption

Interfacial Behavior of Protein Mutants and Variants

Orientation and Activity of Immobilized Antibodies

Interactions Between Surfaces Coated with Carbohydrates, Glycolipids, and Glycoproteins

Protein Adsorption Kinetics

Mobility of Biomolecules at Interfaces

Protein Adsorption in Relation to Solution Association and Aggregation

Mechanism of Interfacial Exchange: Phenomena for Proteins Adsorbed at Solid—Liquid Interfaces

Interactions Between Proteins and Surfactants at Solid Interfaces

Toward Functionalized Polyelectrolyte Biofilms

Self-Assembly of Functional Protein Multilayers: From Planar Films to Microtemplate Encapsulation

Biological Activity of Adsorbed Proteins

Protein Interactions with Monolayers at the Air—Water Interface

Methods:

Local and Global Optical Spectroscopic Probes of Adsorbed Proteins

Proteins on Surfaces: Methodologies for Surface Preparation and Engineering Protein Function

Studies on the Conformation of Adsorbed Proteins with the Use of Nanoparticle Technology

Scanning Calorimetry in Probing the Structural Stability of Proteins at Interfaces

Ellipsometry and Reflectometry for Studying Protein Adsorption

Surface Plasmon Resonance Spectroscopies for Protein Binding Studies at Functionalized Surfaces

Neutron Reflection Study of Protein Adsorption at the Solid—Solution Interface

XPS, ToF-SIMS, and MALDI-MS for Characterizing Adsorbed Protein Films

Applications:

Interaction of Proteins with Polymeric Synthetic Membranes

Protein Adsorption in Intravenous Drug Delivery

Control of Protein Adsorption in Solid-Phase Diagnostics and Therapeutics

Protein Interfacial Behavior in Microfabricated Analysis Systems and Microarrays

Protein Adsorption in the Oral Environment

Adsorbed Biopolymers: Behavior in Food Applications

Index